منابع مشابه
Phytoene desaturase from Arabidopsis.
Carotenoids are isoprenyl pigments necessary for protection of the photosynthetic apparatus in a11 green plants. One of the intermediate steps in carotenoid biosynthesis is the conversion of phytoene to {-carotene via two dehydrogenation reactions catalyzed by the enzyme PDS. We have previously cloned cDNAs for this enzyme from soybean (Glycine max; Bartley et al., 1991) and tomato (Lycopersico...
متن کاملBleaching herbicide flurtamone interferes with phytoene desaturase.
The mode of action of the furanone herbicide flurtamone and derivatives was investigated with cress seedlings and with the unicellular cyanobacterium Anacystis. Either in the light or in the dark these compounds inhibited the formation of alpha- and beta-carotene and all of the xanthophylls in the seedlings. Instead, phytoene, a precursor of colored carotenoids, was accumulated. In illuminated ...
متن کاملPlant-type phytoene desaturase: Functional evaluation of structural implications
Phytoene desaturase (PDS) is an essential plant carotenoid biosynthetic enzyme and a prominent target of certain inhibitors, such as norflurazon, acting as bleaching herbicides. PDS catalyzes the introduction of two double bonds into 15-cis-phytoene, yielding 9,15,9'-tri-cis-ζ-carotene via the intermediate 9,15-di-cis-phytofluene. We present the necessary data to scrutinize functional implicati...
متن کاملEfficient CRISPR/Cas9 Genome Editing of Phytoene desaturase in Cassava
CRISPR/Cas9 has become a powerful genome-editing tool for introducing genetic changes into crop species. In order to develop capacity for CRISPR/Cas9 technology in the tropical staple cassava (Manihot esculenta), the Phytoene desaturase (MePDS) gene was targeted in two cultivars using constructs carrying gRNAs targeting two sequences within MePDS exon 13. After Agrobacterium-mediated delivery o...
متن کاملPhytoene Desaturase from Oryza sativa: Oligomeric Assembly, Membrane Association and Preliminary 3D-Analysis
Recombinant phytoene desaturase (PDS-His6) from rice was purified to near-homogeneity and shown to be enzymatically active in a biphasic, liposome-based assay system. The protein contains FAD as the sole protein-bound redox-cofactor. Benzoquinones, not replaceable by molecular oxygen, serve as a final electron acceptor defining PDS as a 15-cis-phytoene (donor):plastoquinone oxidoreductase. The ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1993
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.103.4.1475